Improved yield of rhEPO in CHO cells with synthetic 5′ UTR
نویسندگان
چکیده
منابع مشابه
Potential roles of 5´ UTR and 3´ UTR regions in post-trans-criptional regulation of mouse Oct4 gene in BMSC and P19 cells
Objective(s):OCT4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. Therefore, the effective expression regulation of this gene is highly critical. UTR regions are of great significance to gene regulation. In this study, we aimed to investigate the potential regulatory role played by 5´UT...
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The treatment of cancer through the induction of natural killer group 2, member D (NKG2D) ligands is of interest, but understanding of mechanisms controlling expression of individual ligand is limited. The major histocompatibility complex (MHC) class I chain related protein B (MICB) is a member of NKG2D ligands. We aimed to investigate the role of 3'-untranslated (3'-UTR) and 5'-untranslated re...
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Background Investigations have shown animal cell cultures’ performance, in terms of cell proliferation and production of recombinant protein, are negatively affected by both lactate’s concentration and its specific production rate. In a previous work, we determined that lactate production was caused by pyruvate accumulation due to its high synthesis rate in the glycolitic pathway and limited co...
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ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2018
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-018-2632-2